3 transplantation of cells isolated from the postnatal retina of the mouse
Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx
... to C-6 of an internal glucose [18] From these results, the structure of the major oligosaccharide in the CO4-3 peak is Glcb1–6Glca1–1aGlc (termed CT3b, Table 4) MALDI-TOF MS analysis of the peracetylated ... to one of the glucoses of the trehalose in either a 1–6 or 1–4 linkage, and the nonreducing glucose linked to the other trehalose glucose in either a 1–6 or a 1–4 linkage To determine the sugar ... enzymes and the sequences obtained from the Mycobacterium tuberculosis genome [24] One of these pathways involves the direct rearrangement of the a1,4-glucosidic linkage of maltose to the a,a1,1...
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Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf
... to the instructions of the kit from Ambion (Austin, TX, USA) The cDNA mix was synthesized from poly(A)+ mRNA using M-MLV reverse transcriptase The cDNA was joined with the adaptor provided by the ... is from the a-ScTXs, the second is from the b-ScTXs Birtoxin is the shortest The depressant and the long-chain excitatory are in the last two lines The right columns indicate percentage of similarities ... to the a-ScTxs (data not shown) However, due to the uniqueness of its sequence, it branches independently of the other a-ScTxs Figure also shows that the core of the three disulfide bridges of...
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Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
... (< 10%) of the GST activity was detected in the flowthrough fraction Further experiments confirmed that the lack of binding was not due to overloading of the column matrix SDS ⁄ PAGE of the affinity-purified ... that the amino acid sequence and ⁄ or the three-dimensional conformation of the enzyme may facilitate catalysis by lowering the activation barrier of the reaction [30] In addition, the rate of ... peptides The length of these peptides was six to eight amino acid residues The deduced amino acid sequence of one Table Substrate specificities of purified cockroach GST compared with those of other...
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Báo cáo khoa học: Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells pot
... 7.54 lm in Hepa-1c1c7 mouse hepatoma cells IxoA was also shown to inhibit the transformation of the murine epidermal JB6 cell with an IC50 of 0.26 lm [23] On the basis of these results, we selected ... measuring the absorbance of formazan at 570 nm The data are mean ± SD from triplicate wells (B) The effects of IxoA, IxoB, PhilB and Withpc on SW480 cells were measured at days (C) The effect of IxoA ... Identification of vacuole content in treated SW480 cells The formation of multiple vacuoles within the cells was observed after treatment of SW480 cells with lM IxoA for 24 h SW480 cells were plated,...
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Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii pdf
... where [S] is the concentration of the sample, EC50 is the effective concentration that causes 50% of the effect, and b represents the cooperativity of the effect Hemagglutination Hamster, mouse and ... variants of a new protein isolated from the skin of the anuran, P sauvagii, whose sequence indicates membership of the Kazal family Most members of this family are small protein inhibitors of serine ... vi is the reaction velocity for each inhibitor concentration, v0 was calculated for each concentration of the inhibitor by nonlinear t of the data from the above negative controls; [I] is the concentration...
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báo cáo hóa học:" Tissue specific characteristics of cells isolated from human and rat tendons and ligaments" pptx
... from different sources, cells from pairs of ACL and PTs isolated from the same subjects and during the same procedure were isolated They were then compared in terms of their proliferation, collagen ... 104 cells in 20 μl of medium 20 μl of the appropriate primary antibody was added to the vial and the cells were then incubated at 4°C for 30 minutes At the end of this time the cells were washed ... levels of progenitor cells and response to dexamethasone Cells from pairs of PT and ACLs were examined for their levels of progenitor cells and their response to a range of concentrations of dexamethasone...
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Application of Bacillus spp. Isolated from the Intestine of Black Tiger Shrimp (Penaeus monodon Fabricius) from Natural Habitat for Control Pathogenic Bacteria in Aquaculture ppt
... MATERIALS AND METHODS Isolation and identification of Bacillus spp Bacillus spp were isolated from the intestine of Penaeus monodon collected from the Gulf of Thailand at Chonburi province Every month, ... determined Morphological change of V harveyi AQVH after colonization V harveyi was isolated from the colonization area, especially from the cross streaking point as well as from the control All samples ... 120 hours for enumeration of the number of bacteria RESULTS Isolation and identification of Bacillus spp From 120 samples of P monodon, 114 strains of genus Bacillus were isolated for antagonistic...
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Báo cáo khoa học: "Histology of two rice bodies isolated from the stifle of an adult draught horse stallion" pps
... noitisoped tnemgip ciredisomeh dna diorec htiw sisorcen dna noitareneged taf fo icof dna ,aisalpatem suonigalitrac raludon fo sucof eno ,illiv suorbif denekciht dah level siht ta enarbmem laivonys ehT ... ,M kisA secnerefeR ”eioS el tnoM fo ertneC esroH naeporuE“ eht rof del tcejorp hcraeser a fo trap siht ot troppus laicnanif eht rof noigeR noollaW eht fo ytilaruR dna erutlucirgA fo yrtsiniM ot ... seitisolliv eht fo esab eht ta yhporta evitucesnoc dna aimehcsi yb decudni si seidob ecir eseht fo noitamrof eht taht tseggus eW aisalpatem fo sngis hcus wohs ton od stnemgarf lardnohcoetso saerehw ,egalitrac...
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Structural and functional characterization of haditoxin, a novel neurotoxin isolated from the venom of ophiohagus hannah (king cobra
... dichroism spectrum of haditoxin Figure 2.8 Comparison of the amino acid sequence of haditoxin with the sequences of other three-finger toxins Figure 2.9 Sequence alignment of haditoxin with the most homologous ... sequences of other three-finger toxins Figure 2.10 Sequence alignment of haditoxin with the sequences of muscarinic toxins and their homologs Figure 2.11 Comparison of the amino acid sequence of haditoxin ... spread among the 700 or so known species of Conus, the roughly 725 species of venomous snakes, the around 1500 species of scorpions, or the Chapter One ca 37,000 known species of spiders, from which...
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A novel protein isolated from the venom of ophiophagus hannah (king cobra) showing beta blocker activity
... for the presence of heat sensing pits between the nostril and the eye (Figure 1.2 C) This gives them the ability of thermal imaging of warm-blooded prey at night Elapidae is a diverse family of ... proteins From the Cys numbers and pattern we concluded that all these new proteins belong to the three-finger toxin family of snake venom proteins We have isolated one of these proteins with a mass of ... are rich sources of enzymes Many of these are hydrolases and they play a role in the digestion of the prey even before the snake swallows it More 10 Table 1.2 Enzymatic proteins from snake venoms...
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Beta cardiotoxin a novel protein isolated from the venom of ophiophagus hannah (king cobra) showing beta blocker activity
... for the presence of heat sensing pits between the nostril and the eye (Figure 1.2 C) This gives them the ability of thermal imaging of warm-blooded prey at night Elapidae is a diverse family of ... proteins From the Cys numbers and pattern we concluded that all these new proteins belong to the three-finger toxin family of snake venom proteins We have isolated one of these proteins with a mass of ... are rich sources of enzymes Many of these are hydrolases and they play a role in the digestion of the prey even before the snake swallows it More 10 Table 1.2 Enzymatic proteins from snake venoms...
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Báo cáo khoa học: A new phospholipase A2 isolated from the sea anemone Urticina crassicornis – its primary structure and phylogenetic classification pptx
... enzyme In the presence of EGTA, it showed no enzymatic activity on either of the substrates (Fig 5) In the presence of NaCl, its enzymatic activity followed the same tendency as that of the group ... A novel PLA2 was purified from the milking exudates of several U crassicornis specimens On the basis of the N-terminal sequence of the isolated protein, its cDNA was isolated, sequenced, and recombinantly ... glycoprotein with a pI of 8.8 The b-form contributes about 70% of the total phospholipase activity of the venom [16,17] A similar PLA2 probably exists in the venom of the related sea anemone...
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Báo cáo khoa học: A new highly toxic protein isolated from the death cap Amanita phalloides is an L-amino acid oxidase pdf
... was isolated from the fruit body of the death cap A phalloides [16] Its physico-chemical, chemical and biological properties differ distinctly from those of the other known toxic proteins of this ... silico tryptic digest of the translated amino acid sequence with the list of masses of tryptic peptides obtained by the MALDI-TOF analysis In total, nine peptides from the list of toxophallin tryptic ... at the beginning of the experiment After 24 h, the tested substances were added at different concentrations The number of viable cells was counted in the hemocytometric chamber The ratio of dead...
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Báo cáo y học: "Infectious salmon anaemia virus (ISAV) isolated from the ISA disease outbreaks in Chile diverged from ISAV isolates from Norway around 1996 and was disseminated around 2005, based on surface glycoprotein gene sequences" pptx
... detection of the Figure the affected by of the outbreaks showing1 the ISA ISA the salmon Chile; A map of Chile Distribution location of outbreaks inaquaculture farms Distribution of the ISA outbreaks ... ISA2in of the X, XI, and total December 01, in Chile distributionRegionsISA outbreaks byof salmon farms 2008 Distributionof the accumulatedXIIin Chile; Chart showing the Distribution of the ISA ... detailed analysis of the new Chile ISAV isolates identifies distinct strains Clade 2.2.2.1.2 consists of the ISAVs from the ISA outbreaks in Chile (48 isolates in total) To further explore the evolutionary...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... and safe Another concern of intravenous transplantation of MSCs is whether the MSCs can migrate into and proliferate in the target tissues, which is the basis of therapeutic effects of MSCs In ... to evaluate the feasibility and safety of intravenous transplantation of stem cells In their study, MSCs were isolated from rhesus monkey and humans in vitro[40] The purified MSCs of passage were ... 1B) The infection rate was over 70% Flow cytometry showed the MSCs had no expressions of CD34, CD45 and HLA-DR, but high expressions of CD29 and CD44 Furthermore, the purity of cells with these...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand RNA being at that time unavailable In the present study we investigated the involvement ... the 341 nt from the 3¢-end of the minus-strand RNA, complementary to the HCV 5¢UTR, folds into six stem loops With the exception of the short SL-A1 stem loop, the one closest to the 3¢-end, these ... that the deletions or the base changes did not alter the structure of the other domains of the (–)IRES RNA as determined by predicted secondary structure with RNA Draw software The structure of the...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008